'Unknown Bacteria\r'

'In ensn be to treat a disease correctly and efficiently, it is serious to first range the microbe that is create the disease. discriminate the bacteria that is causing the disease and using an antibiotic drug that specifically tar foreshortens that microbe, can help prevent or reduce the overuse of broad-spectrum antibiotics-which can lead to antibiotic resistance. When trying to determine the type of microbe that is causing a disease, there are a add of biochemical shields that are administered.The results of these ifferent quizs are analyzed against the characteristics of diametric bacteria and the perpetrator is revealed. Method I subjected my unfamiliar bacteria to 7 different biochemical tests in hostel to identify it. I first coiffureed a streak plate in order to get an isolated colony that I could inoculate and perform the tests on. The Sulfur, Indole and Motility test (SIM), the Glucose Fermentation test and the urea test to help identify my uncharted bacteri a.For the SIM test, I aseptically inoculated the bacteria into a tube of the SIM media and allow it grizzle until the next class period. I took the tube appear of the incubator and irst spy it for the presence of atomic number 16 as well as for motility. I then added 4 drops of Kovacs reagent to the SIM agar deep and observed the reaction. In this test, there are more than one enzyme and substrate involved. The sulfur part of this test the enzymes involved are Cysteine Desulfurase and thiosulfate reductase. Cysteine and thiosulfate are the available substrates that are in the media.If either enzyme is produced by the bacteria being inoculated, Hydrogen Sulfide result be produced and go away combine with the Iron in the Ferrous sulfate that is already in the media and pass on roduce a black return-which is a incontrovertible result. No business ofa black precipitate is a controvert result. In the Indole part of this test, the enzyme involved is tryptophanase has been prod uced, I added Kovacs reagent to the media and observed it for a red color-which would indicate a irresponsible result. No appearance of red would be a banish result.A nonher test I used to identify my unknown was the Glucose Fermentation test. This test determines whether the bacteria produces an acrid, acid and gas or an alcohol. For this test, I inoculated my bacteria into a tube of Glucose broth that contained a shorthorn tube. If, after(prenominal) incubation, there is a bubble in the Durham tube, that is a positive result for the production of gas. No bubble would be a negative result. The media in this tube contained the exponent Phenol Red. A positive result for fermentation to an acid is a yellowness color. A negative result for acid production is red.The Urea test was another test I performed to try and identify my bacteria. For this test I inoculated my unknown into a tube of urea broth. I let them incubate until the next class period. Urease is the enzyme involved i n this test that breaks down urea. Phenol red is the indicator used in this test in order to etect a change in PH. If the pH is slight than 7, the Phenol Red is yellow-which is a negative result. If the pH is greater than 7, that indicates the presence of the enzyme urease and the Phenol Red will be hot pick apart-which is a positive result. Forrest & angstrom unit; Elliott, 2012) Results Test/Culture Escherichia coli Proteus vulgaris Klebsiella pneumonia Shigella flexneri Salmonella typhimurium Unknown #9 Sulfur Indole +1- Motility Methyl Red Voges-Proskauer turn Glucose Lactose Urea Phenylalanine Deaminase My unknown bacteria did not give off a black precipitate in the Sulfur portion of the SIM test so that was a negative result. It turned a pink color in the Indole portion of the SIM test so that was a positive result. The SIM media was cloudy around the celestial orbit where I inoculated the bacteria, indicating it is positive for motility.The Methyl Red test produced an ora nge color, which was a negative result. The VP part of the MR-VP test produced an orange color as well, which is a negative result. There was no growth on the Simmons turn media, indicating a negative result for that test. On twain the Glucose and Lactose test, the broth was yellow and there was a bubble, which indicated that the bacteria produced and acid and a gas.\r\n'