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The analysis of Chromosome Mapping stained chromosomes is the main activity of the keep an eye on called cytogenetics. deoxyribonucleic acid stains illicit a reproducible banding word of advice on chromosomes Quinacrine and Giemsa: DNA dye- chromosome specific pattern Has allowed to for routine of regions in a chromosome p and q arms and different regions Quinacrine, a chemic relative of the antimalarial drug quinine, was wizard of the initial of these more discriminating reagents. Chromosomes that ca-ca been stained with mepacrine gift a characteristic pattern of bright bands on a darker background. However, because quinacrine is a fluorescent compound, the bands appear all when the chromosomes are loose to ultraviolet (UV) light. Ultraviolet irradiation causes some of the quinacrine molecules that have inserted into the chromosome to emit energy. Excellent nonfluorescent staining techniques have likewise been developed. The close to popular of these u ses Giemsa stain, a mixture of dyes named after its inventor, Gustav Giemsa. resembling quinacrine, Giemsa creates a reproducible pattern of bands on each chromosome. 4 ^^^^^Chromosome painting. Probesmade from human DNA have been applied to aspread of human chromosomes.

for each one probe hasbeen designate with a dye that fluoresces a differentcolor ( tip or bright grand) to chance on where Fragments are a specific complementary color sequence .. pink is the centromere, green is the sequence specific to three chromosomes DNA sequences complementary to these probesare dictated in the chromosomes. The pink prob etargets DNA in the centromere of each chro! mosome,whereas the bright green probe targets DNA in only three pairs of chromosomes. Treat mitotic spreads with DNA fragments labelled with flurochromes-Any of a convention of fluorescent dyes used to stain biological specimens. Chromosome Variation A karyotype shows the duplicated chromosomes of a cell arranged for cytogenetic analysis (46 xx Euploid- one set of chromosomes Polyploid-...If you want to get a abundant essay, ordinate it on our website: OrderCustomPaper.com

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